Product Name: ULK1
Product Number: AB-NK298-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Unc 51 (C. elegans) like kinase 1
Target Alias: Unc-51 like kinase 1 (C. elegans); Autophagy related protein 1 homologue; Autophagy-related protein 1 homologue; FLJ38455; Serine/threonine-protein kinase ULK1; Unc51.1; ATG1A; UNC51; C. elegans; homologue of; EC:2.7.11.1; Serine/threonine protein kinase Unc51.1; FLJ46475; ATG 1; hATG1; ATG1; Unc-51-like kinase 1; Serine/threonine protein kinase ULK1; ULK1; Unc 51 like kinase 1; KIAA0722; UNC 51; ULK 1; ULK1_HUMAN; UNC51; ATG1 autophagy related 1 homologue;
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK298-2
Antibody Target Type: Pan-specific
Protein UniProt: O75385
Protein SigNET: O75385
Target Alias: Unc-51 like kinase 1 (C. elegans); Autophagy related protein 1 homologue; Autophagy-related protein 1 homologue; FLJ38455; Serine/threonine-protein kinase ULK1; Unc51.1; ATG1A; UNC51; C. elegans; homologue of; EC:2.7.11.1; Serine/threonine protein kinase Unc51.1; FLJ46475; ATG 1; hATG1; ATG1; Unc-51-like kinase 1; Serine/threonine protein kinase ULK1; ULK1; Unc 51 like kinase 1; KIAA0722; UNC 51; ULK 1; ULK1_HUMAN; UNC51; ATG1 autophagy related 1 homologue;
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK298-2
Antibody Target Type: Pan-specific
Protein UniProt: O75385
Protein SigNET: O75385
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the mid-protein of Human ULK1 (AA 567-577)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the mid-protein of Human ULK1 (AA 567-577)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium-weak ~95 kDa protein band in rat brain lysates, and a medium ~113 kDa protein band in HeLa cell lysates.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium-weak ~95 kDa protein band in rat brain lysates, and a medium ~113 kDa protein band in HeLa cell lysates.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
Related Product 1: ULK2 pan-specific antibody (Cat. No.: AB-NK354-1)
Related Product 2: ULK3 pan-specific antibody (Cat. No.: AB-NK355-1)
Related Product 2: ULK3 pan-specific antibody (Cat. No.: AB-NK355-1)
Scientific Background: UNC-51 like kinase 1 (ULK1) is widely expresed and contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. It has been linked to axon growth and is essential for autophagy. Structurally, ULK1 is similar to ATG1, and it appears that both Atg1/ULK1 can bind to several ATG proteins regulating phosphorylation states and protein trafficking.
References
[1] Okazaki N. et al. (2000) Brain Res Mol Brain Res. 85: 1-12.
[2] Young A.R. et al. (2006) J Cell Sci. 119: 3888-900.
[3] Kamada Y. et al. (2000) J Cell Biol. 150: 1507-13.
[4] Lee S.B et al. (2007) EMBO Rep. 8: 360-5.
[5] Hara T. et al. (2008) J Cell Biol. 181: 497-510.
[1] Okazaki N. et al. (2000) Brain Res Mol Brain Res. 85: 1-12.
[2] Young A.R. et al. (2006) J Cell Sci. 119: 3888-900.
[3] Kamada Y. et al. (2000) J Cell Biol. 150: 1507-13.
[4] Lee S.B et al. (2007) EMBO Rep. 8: 360-5.
[5] Hara T. et al. (2008) J Cell Biol. 181: 497-510.
© Kinexus Bioinformatics Corporation 2017