Product Name: PCTK2-ANT
Product Number: AB-NK285-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Cell division protein kinase 17; Protein-serine kinase PCTAIRE-2
Target Alias: CDK17; KPT2; PCTAIRE protein kinase 2; PCTAIRE-motif protein kinase 2; PCTK2; Serine/threonine-protein kinase PCTAIRE-2
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK285-1
Antibody Target Type: Pan-specific
Protein UniProt: Q00537
Protein SigNET: Q00537
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCTK2 (PCTAIRE2, CDK17) sequence peptide Cat. No.: PE-01BEJ85
Target Alias: CDK17; KPT2; PCTAIRE protein kinase 2; PCTAIRE-motif protein kinase 2; PCTK2; Serine/threonine-protein kinase PCTAIRE-2
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK285-1
Antibody Target Type: Pan-specific
Protein UniProt: Q00537
Protein SigNET: Q00537
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCTK2 (PCTAIRE2, CDK17) sequence peptide Cat. No.: PE-01BEJ85
Antibody Immunogen Sequence: CGSQTIDESLSELAEQMTIE
Antibody Immunogen Description: Corresponds to amino acid residues G14 to E32; Post-N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues G14 to E32; Post-N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in mouse testes.
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in mouse testes.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-65 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for medium ~75 and ~30 kDa proteins. Strong ~ 30 kDa cross-reactive protein was also detected in mouse thymus as well as a weaker ~48 kDa protein.
Related Product 1: PCTK2-ANT blocking peptide
Related Product 2: PCTK2-pS180 phosphosite-specific antibody (Cat. No.: AB-PK756)
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for medium ~75 and ~30 kDa proteins. Strong ~ 30 kDa cross-reactive protein was also detected in mouse thymus as well as a weaker ~48 kDa protein.
Related Product 1: PCTK2-ANT blocking peptide
Related Product 2: PCTK2-pS180 phosphosite-specific antibody (Cat. No.: AB-PK756)
Scientific Background: PCTK2 (PCTAIRE2, CDK17) is a protein-serine/threonine kinase of the CMGC group and CDK family. It functions in terminally differentiated neurons. It is also identified to be associated with circulating phospholipid and sphingolipid concentrations. This kinase likely phosphorylates histone H1. Insertional mutagenesis studies in mice support a role for PCTK2 in mouse cancer oncogenesis.
Figure 1. Dot blotting PCTK2-ANT antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017