Product Name: MLK4-CT
Product Number: AB-NK280-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Mixed-lineage protein-serine kinase 4
Target Alias: M3KL4; Mixed lineage kinase 4; KIAA1804
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK280-1
Antibody Target Type: Pan-specific
Protein UniProt: Q5TCX8
Protein SigNET: Q5TCX8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLK4 sequence peptide Cat. No.: PE-01BEA90
Antibody Immunogen Sequence: CMRSKTSRPSIYELEKEFLS
Target Alias: M3KL4; Mixed lineage kinase 4; KIAA1804
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK280-1
Antibody Target Type: Pan-specific
Protein UniProt: Q5TCX8
Protein SigNET: Q5TCX8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLK4 sequence peptide Cat. No.: PE-01BEA90
Antibody Immunogen Sequence: CMRSKTSRPSIYELEKEFLS
Antibody Immunogen Description: Corresponds to amino acid residues M1018 to S1036; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-120 kDa.
Antibody Specificity: Very high
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-120 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells. No significant cross-reactive proteins detected in diverse mouse tissues, except a medium ~30 kDa protein in mouse brain.
Related Product 1: MLK4-CT blocking peptide
Related Product 1: MLK4-CT blocking peptide
Scientific Background: MLK4 (M3KL4) is a protein-serine/threonine kinase of the TKL group and MLK family. It acts to impair TLR4 signalling, and will not activate any of the JNK1/MAPK8, or p38/MAPK14, or ERK2/MAPK1 pathways. Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. Mutations in MLK4 have been linked to Colorectal cancer (CRC).
© Kinexus Bioinformatics Corporation 2017