Product Name: MLK1-CT
Product Number: AB-NK278-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Mixed-lineage protein-serine kinase 1; Mitogen-activated protein kinase kinase kinase 9
Target Alias: M3K9; MAPKKK9; MAP3K9; MLK-1; PRKE1; ENSG00000006432
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK278-1
Antibody Target Type: Pan-specific
Protein UniProt: P80192
Protein SigNET: P80192
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLK1 (MAP3K9) sequence peptide Cat. No.: PE-01BDX, PE-01BDY
Target Alias: M3K9; MAPKKK9; MAP3K9; MLK-1; PRKE1; ENSG00000006432
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK278-1
Antibody Target Type: Pan-specific
Protein UniProt: P80192
Protein SigNET: P80192
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLK1 (MAP3K9) sequence peptide Cat. No.: PE-01BDX, PE-01BDY
Antibody Immunogen Sequence: CAELNTHRPAPYEIQQEFWS
Antibody Immunogen Description: Corresponds to amino acid residues A1086 to S1104; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues A1086 to S1104; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-120 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus, or in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells.
Related Product 1: MLK1-CT blocking peptide
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus, or in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells.
Related Product 1: MLK1-CT blocking peptide
Scientific Background: MLK1 (MAP3K9) is a protein-serine/threonine kinase of the TKL group and MLK family. It links to MAP kinases and will mediate activation of MKK/JNK via MAP2K4/MKK4 and MAP2K7/MKK7. Phosphorylation of T304, S308 and T312 increases MLK1's phosphotransferase activity. A mild effect on threonine phosphorylation and a minor impairment of JNK signalling can occur with a T305A mutation. Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. The MKK/JNK pathway is involved in mediating release of cytochrome c from the mitochondria and inducing cell death via apoptosis. The MKK/JNK signalling pathway regulates stress response via activator protein-1 (Jun) and GATA4 transcription factors. It also plays a role in mitochondrial death signalling pathway, including the release cytochrome c, leading to cell death via apoptosis. MLK1 may be an oncoprotein (OP). In non-small-cell lung cancers, a gain of function has been observed with MLK1 (MAP3K9), which results in downstream activation of ERK1 and ERK2 MAP kinases.
© Kinexus Bioinformatics Corporation 2017