Product Name: MARK2-ANT
Product Number: AB-NK275-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 2
Target Alias: ELKL motif kinase 1; EMK; EMK1; Kinase MARK2; MAP/microtubule affinity-regulating kinase 2; MGC99619; PAR-1; Par1b; PAR-1b; Serine/threonine kinase
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK275-1
Antibody Target Type: Pan-specific
Protein UniProt: Q7KZI7
Protein SigNET: Q7KZI7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MARK2 sequence peptide Cat. No.: PE-01BDR80
Target Alias: ELKL motif kinase 1; EMK; EMK1; Kinase MARK2; MAP/microtubule affinity-regulating kinase 2; MGC99619; PAR-1; Par1b; PAR-1b; Serine/threonine kinase
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK275-1
Antibody Target Type: Pan-specific
Protein UniProt: Q7KZI7
Protein SigNET: Q7KZI7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MARK2 sequence peptide Cat. No.: PE-01BDR80
Antibody Immunogen Sequence: PTLGHLDSKPSSKSNMIRGC
Antibody Immunogen Description: Corresponds to amino acid residues P19 to G37; Post-N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues P19 to G37; Post-N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-90 kDa.
Related Product 1: MARK2-ANT blocking peptide
Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Related Product 3: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK694)
Related Product 5: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Related Product 6: MARK2-BCT pan-specific antibody (Cat. No.: AB-NK275-2)
Related Product 7: MARK3-ANT pan-specific antibody (Cat. No.: AB-NK276-1)
Related Product 8: MARK3-BCT pan-specific antibody (Cat. No.: AB-NK276-2)
Related Product 1: MARK2-ANT blocking peptide
Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Related Product 3: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK694)
Related Product 5: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Related Product 6: MARK2-BCT pan-specific antibody (Cat. No.: AB-NK275-2)
Related Product 7: MARK3-ANT pan-specific antibody (Cat. No.: AB-NK276-1)
Related Product 8: MARK3-BCT pan-specific antibody (Cat. No.: AB-NK276-2)
Related Product 9: MARK3-pT507 phosphosite-specific antibody (Cat. No.: AB-PK697)
Related Product 10: MARK4-ANT pan-specific antibody (Cat. No.: AB-NK277-1)
Related Product 10: MARK4-ANT pan-specific antibody (Cat. No.: AB-NK277-1)
Scientific Background: MARK2 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is activated by phosphorylation on T208 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It mediates cell polarity and microtubule function. Some substrate proteins that MARK2 phosphorylates include CRTC2/TORC2, HDAC7, DCX, KIF13B, MAPT/TAU, MAP2, MAP4, and RAB11FIP2. Phosphorylation of microtubule associated proteins leads to protein dissociation and microtubule degeneration. The phosphorylation of MARK2 by the LKB1/LKB1 complex can occur with a T208A mutation in MARK2. The ability of MARK2 to bind YWHAZ and to dissociate from the membrane occurs with a T596A mutation. Inhibition of MARK2 via small inhibitory RNA induced axon-like outgrowth from neurites.
© Kinexus Bioinformatics Corporation 2017