Product Name: ITK-BCT
Product Number: AB-NK274-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Tyrosine-protein kinase ITK/TSK
Target Alias: EMT; Lyk; PSCTK2; MGC126257; MGC126258; TLK; TSK; CCDS4336.1; ENSG00000113263
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK274-1
Antibody Target Type: Pan-specific
Protein UniProt: Q08881
Protein SigNET: Q08881
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ITK sequence peptide Cat. No.: PE-01BDK85
Antibody Immunogen Sequence: CWKERPEDRPAFSRLLRQLA
Target Alias: EMT; Lyk; PSCTK2; MGC126257; MGC126258; TLK; TSK; CCDS4336.1; ENSG00000113263
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK274-1
Antibody Target Type: Pan-specific
Protein UniProt: Q08881
Protein SigNET: Q08881
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ITK sequence peptide Cat. No.: PE-01BDK85
Antibody Immunogen Sequence: CWKERPEDRPAFSRLLRQLA
Antibody Immunogen Description: Corresponds to amino acid residues C594 to A613; Pre-C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 68-72 kDa.
Related Product 1: ITK-BCT blocking peptide
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 68-72 kDa.
Related Product 1: ITK-BCT blocking peptide
Scientific Background: ITK is a protein-serine/threonine kinase that is a member of the TK group of protein kinases in the Tec family. This kinase is widely distributed from moderate to high levels in most human tissues with highest expression in bladder, lung, lymph node, spleen, stomach, thymus, tonsils and uterus. Ligation of CD2, TCR or CD28 induces activation and tyrosine phosphorylation of ITK at Y512. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK require prior activation of Lck, ZAP70 and PI3-kinase for efficient activation. ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signalling events involved in cytoskeletal reorganization. ITK has been linked with the development of gastric adenocarcinomas, melanoma (metastatic) and lymphoproliferative syndrome ebv-associated autosomal type 1 (LPSA1).
© Kinexus Bioinformatics Corporation 2017