Product Name: HIPK2-BCT
Product Number: AB-NK272-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Homeodomain-interacting protein-serine kinase 2

Target Alias: Homeodomain interacting protein kinase 2; Kinase HIPK2; NBAK1; Nuclear body associated kinase 1; Sialophorin tail associated nuclear serine,threonine kinase; Sialophorin tail associated nuclear serine/threonine kinase; STANK; PRO0593; Q9H2X6; ENSG00000064393

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK272-1

Antibody Target Type: Pan-specific

Protein UniProt: Q9H2X6

Protein SigNET: Q9H2X6

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human HIPK2 sequence peptide Cat. No.: PE-01BDD99

Antibody Immunogen Sequence: HPSQYPAQFAHQTYISASC

Antibody Immunogen Description: Corresponds to amino acid residues H1159 to S1176; Pre-C-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-125 kDa.

Related Product 1: HIPK2-BCT blocking peptide

Related Product 2: HIPK1-pY352 phosphosite-specific antibody (Cat. No.: AB-PK654)

Related Product 3: DYRKtide KinSub - DYR peptide substrate

Scientific Background: HIPK2 is a protein-serine/threonine kinase of the CMGC group and DYRK family. It is believed to be involved in inducing apoptosis, through phosphorylation of p53, after genotoxic stress. It interacts with numerous transcription factors (such as p53, AML1(RUNX1), PAX6, c-MYB or NK3) as well as transcriptional regulators (such as CBP, p300, Groucho, CtBP, HMGA1 or Smads). In this way HIPK2 can activate or repress transcription and thereby influence differentiation, development and the DNA damage response. HIPK2 can promote the apoptotic program via p53-dependent and -independent pathways through phosphorylation of p53 at S46 or phosphorylation of the anti-apoptotic co-repressor CtBP at S422 (both actions leading to the transcription of pro-apoptotic target genes). HIPK2 may be an oncoprotein (OP) or a tumour suppressor protein (TSP). HIPK2 is upregulated in some cervical cancers, and down regulated in some thyroid and breast carcinomas. In most cancer systems appears to repress growth and prevent tumourigenesis. In cervical cancer was described to be upregulated but this may represent a compensation of normal cellular machinery.