Product Name: Frk-ANT
Product Number: AB-NK269-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Fyn-related kinase; Tyrosine-protein kinase Frk
Target Alias: Nuclear tyrosine protein kinase RAK; PTK5; RAK; GTK; CCDS5103.1; ENSG00000111816
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK269-1
Antibody Target Type: Pan-specific
Protein UniProt: P42685
Protein SigNET: P42685
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Frk sequence peptide Cat. No.: PE-01BCX90
Antibody Immunogen Sequence: LSTEADKSTVIENPGALC
Target Alias: Nuclear tyrosine protein kinase RAK; PTK5; RAK; GTK; CCDS5103.1; ENSG00000111816
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK269-1
Antibody Target Type: Pan-specific
Protein UniProt: P42685
Protein SigNET: P42685
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Frk sequence peptide Cat. No.: PE-01BCX90
Antibody Immunogen Sequence: LSTEADKSTVIENPGALC
Antibody Immunogen Description: Corresponds to amino acid residues L19 to L35; Post-N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.
Related Product 1: Frk-ANT blocking peptide
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.
Related Product 1: Frk-ANT blocking peptide
Related Product 2: Frk-NT pan-specific antibody (Cat. No.: AB-NK269-2)
Related Product 3: Frk-pY387 phosphosite-specific antibody (Cat. No.: AB-PK641)
Related Product 4: Frk-pY497 phosphosite-specific antibody (Cat. No.: AB-PK642)
Related Product 3: Frk-pY387 phosphosite-specific antibody (Cat. No.: AB-PK641)
Related Product 4: Frk-pY497 phosphosite-specific antibody (Cat. No.: AB-PK642)
Scientific Background: Frk is a protein-tyrosine kinase of the TK group and Src family that is found in the nucleus.. It is involved in positive regulation of PTEN by increasing its stability and preventing degradation and negative regulation of cell proliferation. It may also have functions during G1 and S phase in the nucleus to suppress growth. Frk may be a tumour suppressor protein (TSP). Cancer-related mutations in human tumours point to a loss of function of the protein kinase. nsertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
© Kinexus Bioinformatics Corporation 2017