Product Name: DMPK2-NT
Product Number: AB-NK265-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Myotonic dystrophy protein kinase 2; Serine-threonine-protein kinase MRCK gamma
Target Alias: MRCKG; Myotonic dystrophy protein kinase like protein ; CDC42BPG; HSMDPKIN
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK265-1
Antibody Target Type: Pan-specific
Protein UniProt: Q6DT37
Protein SigNET: Q6DT37
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DMPK2 (MRCK-g) sequence peptide Cat. No.: PE-01BCS80
Target Alias: MRCKG; Myotonic dystrophy protein kinase like protein ; CDC42BPG; HSMDPKIN
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK265-1
Antibody Target Type: Pan-specific
Protein UniProt: Q6DT37
Protein SigNET: Q6DT37
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DMPK2 (MRCK-g) sequence peptide Cat. No.: PE-01BCS80
Antibody Immunogen Sequence: MERRLRALEQLARGEAGGC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to C19; N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues M1 to C19; N-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around n/a kDa.
Related Product 1: DMPK2-NT blocking peptide
Related Product 2: DMPKSubtide - DMPK1 (DMPK) protein kinase substrate peptide
Related Product 1: DMPK2-NT blocking peptide
Related Product 2: DMPKSubtide - DMPK1 (DMPK) protein kinase substrate peptide
Scientific Background: DMPK2 is a protein-serine/threonine kinase of the AGC group and DMPK family. It is a protein kinase that is hypothesized to function as a downstream effector of CDC42 during cytoskeletal rearrangement. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation. It is maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. DMPK2 also plays a role in the regulation of actin-myosin contractility through the modulation of MYPT1 and MLC2 activity, which are required for cell invasion and motility. In addition, mutations in the DMPK2 gene have been associated with myotonic dystrophy. The DMPK2 protein is highly expressed in heart and skeletal muscle, with little to no expression in the brain, placenta, lung, liver, kidney, and pancreas. The DMPK2 gene maps to chromosome 11q13.
© Kinexus Bioinformatics Corporation 2017