Product Name: DMPK1-AKCD
Product Number: AB-NK264-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Myotonic dystrophy protein kinase
Target Alias: Myotonic dystrophy protein kinase; MDPK; DM-kinase; DMK; DMPK; MT-PK
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK264-1
Antibody Target Type: Pan-specific
Protein UniProt: Q09013
Protein SigNET: Q09013
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DMPK1 (DMPK) sequence peptide Cat. No.: PE-01BCR70
Target Alias: Myotonic dystrophy protein kinase; MDPK; DM-kinase; DMK; DMPK; MT-PK
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK264-1
Antibody Target Type: Pan-specific
Protein UniProt: Q09013
Protein SigNET: Q09013
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DMPK1 (DMPK) sequence peptide Cat. No.: PE-01BCR70
Antibody Immunogen Sequence: CMALRDSEVPGPTPMELEA
Antibody Immunogen Description: Corresponds to amino acid residues C407 to A425; Just after kinase catalytic domain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues C407 to A425; Just after kinase catalytic domain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-75 kDa.
Related Product 1: DMPK1-AKCD blocking peptide
Related Product 2: DMPKSubtide - DMPK1 (DMPK) protein kinase substrate peptide
Related Product 1: DMPK1-AKCD blocking peptide
Related Product 2: DMPKSubtide - DMPK1 (DMPK) protein kinase substrate peptide
Scientific Background: DMPK1 is a protein-serine/threonine kinase of the AGC group and DMPK family. It is a protein kinase that is essential for maintaining skeletal muscle structure and function. It is activated in response to G protein second messengers. Proteolytic processing of the C-terminus may remove the transmembrane domain and release the kinase from membranes stimulating its activity. Phosphorylation by Raf1 may result in activation of DMPK. It is maintained in an inactive conformation by the negative autoregulatory C-terminal coiled-coil region.
© Kinexus Bioinformatics Corporation 2017