Target Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2

Target Alias: AAPK2; AMPK alpha-2 chain; Kinase AMPK-alpha2; AMPK2; PRKAA; PRKAA2 ENSG00000162409

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK260-2

Antibody Target Type: Pan-specific

Protein UniProt: P54646

Protein SigNET: P54646

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human AMPKa2 (PRKAA2) sequence peptide Cat. No.: PE-01BCE60

Antibody Immunogen Sequence: CTESEVMNSLYSGDPQDQL

Antibody Immunogen Description: Corresponds to amino acid residues C302 to L320; After catalytic domain in region not conserved with AMPKa1

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human