Product Name: YSK4-2
Product Number: AB-NK256-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: SPS1/STE20-related protein kinase YSK4; Mitogen-activated protein kinase kinase kinase 8
Target Alias: FLJ23074; Rck; Regulated in COPD, protein kinase; YSK4; YSK4 Sps1/Ste20-related kinase
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK256-2
Antibody Target Type: Pan-specific
Protein UniProt: Q56UN5
Protein SigNET: Q56UN5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human YSK4 (MAP3K19) sequence peptide Cat. No.: PE-01AZL90
Target Alias: FLJ23074; Rck; Regulated in COPD, protein kinase; YSK4; YSK4 Sps1/Ste20-related kinase
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK256-2
Antibody Target Type: Pan-specific
Protein UniProt: Q56UN5
Protein SigNET: Q56UN5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human YSK4 (MAP3K19) sequence peptide Cat. No.: PE-01AZL90
Antibody Immunogen Sequence: CSALQLLKHSFLERSH
Antibody Immunogen Description: Corresponds to amino acid residues S1314 to H1328; Kinase last alpha-chain + C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues S1314 to H1328; Kinase last alpha-chain + C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak reactivity with recombinant human TYK2 on protein dot blots.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak reactivity with recombinant human TYK2 on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-70 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEKK2 and MEKK6.
Related Product 1: YSK4-2 blocking peptide
Related Product 2: YSK4-1 pan-specific antibody (Cat. No.: AB-NK256-1)
Related Product 3: YSK4-3 pan-specific antibody (Cat. No.: AB-NK256-3)
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-70 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEKK2 and MEKK6.
Related Product 1: YSK4-2 blocking peptide
Related Product 2: YSK4-1 pan-specific antibody (Cat. No.: AB-NK256-1)
Related Product 3: YSK4-3 pan-specific antibody (Cat. No.: AB-NK256-3)
Scientific Background: YSK4 (MAP3K19) is a protein-serine/threonine kinase of the STE group and STE11 family.
Figure 1. Dot blotting YSK4-2 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017