Product Name: WNK4-3
Product Number: AB-NK255-3
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Serine/threonine-protein kinase WNK4
Target Alias: Kinase WNK4; PRKWNK4; Protein kinase with no lysine 4; Protein kinase, lysine-deficient 4; WNK lysine deficient protein kinase 4; PHA2B; CCDS11439.1; ENSG00000126562
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK255-3
Antibody Target Type: Pan-specific
Protein UniProt: Q96J92
Protein SigNET: Q96J92
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK4 (PRKWNK4) sequence peptide Cat. No.: PE-01AZJ99
Target Alias: Kinase WNK4; PRKWNK4; Protein kinase with no lysine 4; Protein kinase, lysine-deficient 4; WNK lysine deficient protein kinase 4; PHA2B; CCDS11439.1; ENSG00000126562
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK255-3
Antibody Target Type: Pan-specific
Protein UniProt: Q96J92
Protein SigNET: Q96J92
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK4 (PRKWNK4) sequence peptide Cat. No.: PE-01AZJ99
Antibody Immunogen Sequence: CRASKGVTFAGDVGRM
Antibody Immunogen Description: Corresponds to amino acid residues R1229 to M1243; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues R1229 to M1243; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110, 140-180 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK4-3 blocking peptide
Related Product 2: WNK4-1 pan-specific antibody (Cat. No.: AB-NK255-1)
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK4-3 blocking peptide
Related Product 2: WNK4-1 pan-specific antibody (Cat. No.: AB-NK255-1)
Scientific Background: WNK4 (PRKWNK4) is a protein-serine/threonine kinase of the Other group and WNK family. It regulates electrolyte homeostasis. WNK4 can activate the genes SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. The renal potassium channel KCNJ1, through a kinase-mediated mechanism, will be inhibited via clathrin-mediated receptor endocytosis. WNK4 can also phosphorylate NEDD4L. Activation of WNK4 requires autophosphorylation of S335. Phosphorylation of S331 also promotes increased activity.
Figure 1. Dot blotting WNK4-3 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017