Product Name: Kit-1
Product Number: AB-NK241-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Mast/stem cell growth factor receptor protein-tyrosine Kit
Target Alias: CD117; C-kit; Kinase Kit; Mast/stem cell growth factor receptor precursor; PBT; SCFR; SL; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homologue; ENSG00000157404
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK241-1
Antibody Target Type: Pan-specific
Protein UniProt: P10721
Protein SigNET: P10721
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Kit sequence peptide Cat. No.: PE-01AUS99
Target Alias: CD117; C-kit; Kinase Kit; Mast/stem cell growth factor receptor precursor; PBT; SCFR; SL; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homologue; ENSG00000157404
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK241-1
Antibody Target Type: Pan-specific
Protein UniProt: P10721
Protein SigNET: P10721
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Kit sequence peptide Cat. No.: PE-01AUS99
Antibody Immunogen Sequence: CSIHPGKSDLIVRVGD
Antibody Immunogen Description: Corresponds to amino acid residues S38 to D52; In the extracellular domain of the receptor.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues S38 to D52; In the extracellular domain of the receptor.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-145 kDa.
Antibody Cross Reactivity: Medium cross-immunoreactivity on protein dot blots with recombinant human CSF1R.
Related Product 1: Kit-1 blocking peptide
Related Product 2: Kit-2 pan-specific antibody (Cat. No.: AB-NK241-2)
Related Product 3: Kit-pY936 phosphosite-specific antibody (Cat. No.: AB-PK673)
Related Product 4: Kit -S821+Y823 phosphosite-specific antibody (Cat. No.: AB-PK674)
Antibody Cross Reactivity: Medium cross-immunoreactivity on protein dot blots with recombinant human CSF1R.
Related Product 1: Kit-1 blocking peptide
Related Product 2: Kit-2 pan-specific antibody (Cat. No.: AB-NK241-2)
Related Product 3: Kit-pY936 phosphosite-specific antibody (Cat. No.: AB-PK673)
Related Product 4: Kit -S821+Y823 phosphosite-specific antibody (Cat. No.: AB-PK674)
Scientific Background: Kit (SCFR) is a protein-tyrosine kinase of the TK group and PDGFR family. It is a receptor kinase that binds SCF/KitLG. Mast,stem cell growth factor (steel factor) binding induces Kit dimerization and autophosphorylation. Protein interactions are induced with phosphorylation at Y568 (with APS, Cbl, SHP2, & Src), Y900 (with Crk & PIK3C2A) and Y936 (with APS, Cbl, Grb2 & Grb7). Phosphorylation of S741 and S746 inhibits the kinase activity of Kit. Kit can mediate cell proliferation, survival, hematopoiesis, gametogenesis, stem cell maintenance, mast cell development, function, and migration, as well as melanogenesis. Some substrates that Kit can phosphorylate include PIK3R1, PLCG1, SH2B2/APS and CBL.This kinase is highly expressed and widely distributed in most tested human tissues. It was first identified as the cellular homologue of the feline sarcoma viral oncogene v-kit. Mutations in Kit are associated with gastrointestinal stromal tumors (GIST), mast cell leukemia, acute myelogenous leukemia (AML), and piebaldism. Kit has also been linked with the development of colorectal adenocarcinomas and melanoma progression. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis.
Figure 1. Dot blotting KIT-1 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017