Product Name: SMG1-1
Product Number: AB-NK233-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Serine/threonine-protein kinase SMG1; Lambda/iota protein kinase C-interacting protein
Target Alias: ATX; KIAA0421; Lambda/iota protein kinase C-interacting protein; LIP
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK233-2
Antibody Target Type: Pan-specific
Protein UniProt: Q96Q15
Protein SigNET: Q96Q15
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SMG1 sequence peptide Cat. No.: PE-01ATG85
Target Alias: ATX; KIAA0421; Lambda/iota protein kinase C-interacting protein; LIP
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK233-2
Antibody Target Type: Pan-specific
Protein UniProt: Q96Q15
Protein SigNET: Q96Q15
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SMG1 sequence peptide Cat. No.: PE-01ATG85
Antibody Immunogen Sequence: CGTKYPRSWNDWQPRT
Antibody Immunogen Description: Corresponds to amino acid residues G18 to T32
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues G18 to T32
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 350-410 kDa.
Antibody Specificity: High
Related Product 1: SMG1-1 blocking peptide
Related Product 2: SMG1 pan-specific antibody (Cat. No.: AB-NK233)
Related Product 3: SMG1-1 pan-specific antibody (Cat. No.: AB-NK233-2)
Related Product 4: SMG1-3 pan-specific antibody (Cat. No.: AB-NK233-3)
Related Product 5: SMG1-pT3550 phosphosite-specific antibody (Cat. No.: AB-PK817)
Antibody Specificity: High
Related Product 1: SMG1-1 blocking peptide
Related Product 2: SMG1 pan-specific antibody (Cat. No.: AB-NK233)
Related Product 3: SMG1-1 pan-specific antibody (Cat. No.: AB-NK233-2)
Related Product 4: SMG1-3 pan-specific antibody (Cat. No.: AB-NK233-3)
Related Product 5: SMG1-pT3550 phosphosite-specific antibody (Cat. No.: AB-PK817)
Scientific Background: SMG1 (ATX) is a protein-serine/threonine kinase of the Atypical group and PIKK family. It mediates the genotoxic stress pathway as well as the mRNA surveillance pathway. SMG1 will target nonsense RNA molecules for degradation via phosphorylation of RENT1/UPF1. SMG1 is essential for optimal phosphorylation and activity of p53/TP53 following genotoxic stress. It is iInhibited by caffeine, LY294002 and wortmannin.
© Kinexus Bioinformatics Corporation 2017