Product Name: MELK
Product Number: AB-NK229-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Maternal embryonic leucine zipper kinase

Target Alias: hMELK; hPK38; KIAA0175; Maternal embryonic leucine zipper kinase; MELK; pEg3 kinase; Protein kinase PK38

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK229-1

Antibody Target Type: Pan-specific

Protein UniProt: Q14680

Protein SigNET: Q14680

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MELK sequence peptide

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human; Mouse; Rat

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-75 kDa.

Related Product 2: MELK pan-specific antibody (Cat. No.: AB-NK229-2P)

Related Product 3: MELK-1 pan-specific antibody (Cat. No.: AB-NK229-2)

Related Product 4: MELK-2 pan-specific antibody (Cat. No.: AB-NK229-3)

Related Product 5: MELK-pY438 phosphosite-specific antibody (Cat. No.: AB-PK701)

Scientific Background: MELK is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It can occur in 8 human isoforms ranging from 74.642 to 52.528 kDa in size. It is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It is activated by autophosphorylation of the T-loop at T167 and S171: in contrast to other members of the SNF1 subfamily, phosphorylation at T167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Phosphorylation at T478 induces interaction with NIPP-1. It exhibits a broad substrate specificity with targets that include BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. It is an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1, and its phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. It phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of its pro-apoptotic function. It inhibits spliceosome assembly during mitosis by phosphorylating ZNF622, which contributes to its redirection to the nucleus. It interacts with ZNF622 and PPP1R8. It is expressed in placenta, kidney, thymus, testis, ovary and intestine, and is up-regulated in many cancers cells.
Figure 1. Immunoblotting of various cell lines with NK229-1 antibody at a 200 X dilution. The position of the full size target protein MELK is indicated. The expected molecular masses of MELK isoforms range from 52.5 to 74.6 kDa. Each lane was loaded with 20 µg of cell lysate protein.