Product Name: Raf-A-CT (RafA-4)
Product Number: AB-NK205
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: A-Raf proto-oncogene serine/threonine-protein kinase
Target Alias: ARaf; A-Raf; ARaf1; A-raf-1; Kinase A-Raf; PKS; PKS2; Proto-oncogene Pks; V-raf murine sarcoma 3611 viral oncogene; RP1-230G1.1-005; RP1-230G1_1; A6NIT1; B4DMG5; Q5H9B2; Q96II5ENSG00000078061
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK205
Antibody Target Type: Pan-specific
Protein UniProt: P10398
Protein SigNET: P10398
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Raf-A (ARaf) sequence peptide
Target Alias: ARaf; A-Raf; ARaf1; A-raf-1; Kinase A-Raf; PKS; PKS2; Proto-oncogene Pks; V-raf murine sarcoma 3611 viral oncogene; RP1-230G1.1-005; RP1-230G1_1; A6NIT1; B4DMG5; Q5H9B2; Q96II5ENSG00000078061
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK205
Antibody Target Type: Pan-specific
Protein UniProt: P10398
Protein SigNET: P10398
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Raf-A (ARaf) sequence peptide
Antibody Immunogen Sequence: PSLHRTQADELPAC
Antibody Immunogen Description: Corresponds to amino acid residues P584 to C597; C-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues P584 to C597; C-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with atarget-sized protein in rat pheochromocytoma (PC12) cells.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with atarget-sized protein in rat pheochromocytoma (PC12) cells.
Antibody Species Reactivity: Human; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 63-70 kDa.
Related Product 1: Raf-A-1 pan-specific antibody (Cat. No.: AB-NK205-1)
Related Product 2: Raf-A-2 pan-specific antibody (Cat. No.: AB-NK205-3P)
Related Product 3: Raf-A-3 pan-specific antibody (Cat. No.: AB-NK205-4P)
Related Product 4: Raf-A-pY302 phosphosite-specific antibody (Cat. No.: AB-PK500)
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 63-70 kDa.
Related Product 1: Raf-A-1 pan-specific antibody (Cat. No.: AB-NK205-1)
Related Product 2: Raf-A-2 pan-specific antibody (Cat. No.: AB-NK205-3P)
Related Product 3: Raf-A-3 pan-specific antibody (Cat. No.: AB-NK205-4P)
Related Product 4: Raf-A-pY302 phosphosite-specific antibody (Cat. No.: AB-PK500)
Scientific Background: A-Raf (RafA) is a protein-serine/threonine kinase of the TKL group and Raf family. It is involved in the transduction of mitogenic signals from the cell membrane receptors via Ras and MEK1/2 to the nucleus. It has been implicated in playing a role in the postsynaptic responses of hippocampal neuron. Phosphorylation of S214, S257, S262, S264, Y301, Y302, S432, T442, T452, T455 and S582 increases phosphotransferase activity. It is inhibited by Nexavar/Sorafenib (IC50>50 nM). A-Raf may be an oncoprotein (OP) based on its similarity to Raf1 and B-Raf. However, it does not display a higher than normal rate of mutation than most proteins in human tumours. The active form of the protein kinase acts to promote tumour cell proliferation.
© Kinexus Bioinformatics Corporation 2017