Product Name: PKG1a-NT (PKG1a-1)
Product Number: AB-NK202
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: cGMP-dependent protein kinase 1, alpha isozyme
Target Alias: cGK 1 alpha; cGKI-alpha; cGKI-beta; cGMP-dependent protein kinase 1, alpha isozyme; DKFZp686K042; FLJ36117; KGP1A; Kinase PKG1; PGK; PKG; PKG1-alpha; Protein kinase, cGMP-dependent, type I
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK202
Antibody Target Type: Pan-specific
Protein UniProt: Q13976
Protein SigNET: Q13976
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKG1a (PRKG1A) sequence peptide
Target Alias: cGK 1 alpha; cGKI-alpha; cGKI-beta; cGMP-dependent protein kinase 1, alpha isozyme; DKFZp686K042; FLJ36117; KGP1A; Kinase PKG1; PGK; PKG; PKG1-alpha; Protein kinase, cGMP-dependent, type I
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK202
Antibody Target Type: Pan-specific
Protein UniProt: Q13976
Protein SigNET: Q13976
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKG1a (PRKG1A) sequence peptide
Antibody Immunogen Sequence: ELEEDFAKILMLKEEC
Antibody Immunogen Description: Corresponds to amino acid residues E3 to E17; N-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues E3 to E17; N-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 77-83 kDa.
Related Product 1: PKG1-BKCD pan-specific antibody (Cat. No.: AB-NK202-2)
Related Product 2: PKG1-pT515+pT517 phosphosite-specific antibody (Cat. No.: AB-PK776)
Related Product 3: PKG1b-NT (PKG1b-1) pan-specific antibody (Cat. No.: AB-NK203)
Related Product 4: PKG1Subtide - PKG1 (PRKG1) protein kinase substrate peptide
Related Product 1: PKG1-BKCD pan-specific antibody (Cat. No.: AB-NK202-2)
Related Product 2: PKG1-pT515+pT517 phosphosite-specific antibody (Cat. No.: AB-PK776)
Related Product 3: PKG1b-NT (PKG1b-1) pan-specific antibody (Cat. No.: AB-NK203)
Related Product 4: PKG1Subtide - PKG1 (PRKG1) protein kinase substrate peptide
Scientific Background: PKG1 (PRKG1) is a protein-serine/threonine kinase of the AGC group and PKG family. It is activated by cGMP, which is generated in response to nitric oxide. Phosphorylation at T59 increases phosphotransferase activity and its affinity for cGMP Phosphorylation at T517 increases phosphotransferase activity. An R177Q mutation in AAT8 results in a constitutively active form of PKG1, which also has reduced cGMP binding.
© Kinexus Bioinformatics Corporation 2017