Product Name: KKIALRE-CT (CDK1L-1)
Product Number: AB-NK199
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Cyclin-dependent kinase-like 1; Serine/threonine protein kinase KKIALRE
Target Alias: Cyclin-dependent kinase-like 1
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK199
Antibody Target Type: Pan-specific
Protein UniProt: Q00532
Protein SigNET: Q00532
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDKL1 sequence peptide
Antibody Immunogen Sequence: CLDNKKYYSDTKKLNYR
Target Alias: Cyclin-dependent kinase-like 1
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK199
Antibody Target Type: Pan-specific
Protein UniProt: Q00532
Protein SigNET: Q00532
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDKL1 sequence peptide
Antibody Immunogen Sequence: CLDNKKYYSDTKKLNYR
Antibody Immunogen Description: Corresponds to amino acid residues L338 to R353; C-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-44 kDa.
Scientific Background: CDKL1 (KKIALRE) is a protein-serine/threonine kinase of the CMGC group and CDKL family. The deduced CDKL1 protein is 358-amino acids long and has 43% sequence identity to CDC2. However, it was identified as a human homologue of cyclin-dependent kinases by low stringency cloning studies. In view of its size and the conservation of a Thr-X-Tyr dual phosphorylation site motif in the region between kinase subdomains VII and VIII, it is more likely that KKAILRE corresponds to a MAP kinase family member. CDKL1 mRNA is expressed in several human tissues, with highest expression levels in the kidney. CDKL1 may be an oncoprotein (OP). Significantly elevated CDKL1 expression was observed in gastric cancer tissue specimens. In addition, loss of CDKL1 function in gastric cancer cell lines (SGC7901 and MGC-803) resulted in impaired cell proliferation and enhanced apoptosis. Furthermore, siRNA-mediated knockdown of CDKL1 expression stimulated the pro-apoptotic factor Bcl-2-interacting killer (BIK) and reduced cellular expression of the proliferating cell nuclear antigen (PCNA), indicating reduced cellular proliferation. Therefore, CDKL1 is thought to play a key role in gastric cancer cell proliferation and survival.
© Kinexus Bioinformatics Corporation 2017