Product Name: Tyk2-3
Product Number: AB-NK181-5
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein-tyrosine kinase 2 (Jak-related)
Target Alias: JTK1; Kinase Tyk2; Tyrosine kinase 2; CCDS12236.1; ENSG00000105397
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK181-5
Antibody Target Type: Pan-specific
Protein UniProt: P29597
Protein SigNET: P29597
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Tyk2 sequence peptide Cat. No.: PE-01AVL80
Antibody Immunogen Sequence: CWETEASFRPTFENLI
Target Alias: JTK1; Kinase Tyk2; Tyrosine kinase 2; CCDS12236.1; ENSG00000105397
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK181-5
Antibody Target Type: Pan-specific
Protein UniProt: P29597
Protein SigNET: P29597
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Tyk2 sequence peptide Cat. No.: PE-01AVL80
Antibody Immunogen Sequence: CWETEASFRPTFENLI
Antibody Immunogen Description: Corresponds to amino acid residues C1151 to I1166; Kinase last alpha-chain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak reactivity with recombinant human Tyk2 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak reactivity with recombinant human Tyk2 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Very weak immunoreactivity on protein dot blots with recombinant human JAK3. No immunoreactivity on protein dot blots with recombinant human JAK1 and JAK2.
Related Product 1: Tyk2-3 blocking peptide
Related Product 2: Tyk2-1 pan-specific antibody (Cat. No.: AB-NK181-3)
Related Product 3: Tyk2-pY1054+pY1055 phosphosite-specific antibody (Cat. No.: AB-PK845)
Antibody Cross Reactivity: Very weak immunoreactivity on protein dot blots with recombinant human JAK3. No immunoreactivity on protein dot blots with recombinant human JAK1 and JAK2.
Related Product 1: Tyk2-3 blocking peptide
Related Product 2: Tyk2-1 pan-specific antibody (Cat. No.: AB-NK181-3)
Related Product 3: Tyk2-pY1054+pY1055 phosphosite-specific antibody (Cat. No.: AB-PK845)
Scientific Background: Tyk2 is a protein-tyrosine kinase of the TK group and JakA family. Phosphorylation of Y1054 and Y1055 increases phosphotransferase activity. Tyk2 mediates intracellular signal transduction from Type I interferon-alpha/beta receptor (IFN) signalling and phosphorylates the interferon-alpha/beta receptor alpha chain. Missense mutations in this gene have been found in colon and ovarian tumours.
Figure 1. Dot blotting TYK2-3 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017