Product Name: PKCb (PKCb-1)

Product Number: AB-NK133-2

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Protein-serine kinase C beta 1

Target Alias: Kinase PKC-beta; KPCB; MGC41878; PKC II; PKC-B; PKC-beta; PKC-II; PRKCB; PRKCB1; PRKCB2; Protein kinase C, beta type

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK133-2

Antibody Target Type: Pan-specific

Protein UniProt: P05771

Protein SigNET: P05771

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PKCb (PRKCB1) sequence peptide Cat. No.:

Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 2.5 mg/ml

Storage Buffer: 100 mM Tris-glycine, pH 7.0

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human; Mouse; Rat

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 75-85 kDa.

Related Product 1: PKCb-pT500 phosphosite-specific antibody (Cat. No.: AB-PK766)

Scientific Background: PKCb (PRKCB, PKC-beta) is a protein-serine/threonine kinase in the classical protein kinase C family. It is calcium activated, and dependent on acidic phospholipids (e.g. phosphatidylserine) and diacylglycerol for full phosphotransferase activity. PKCb can bind 3 calcium ions for each subunit. Phosphorylation of T635 and T642 is critical for kinase activity. Phosphorylation of S661 inhibits membrane binding and causes release into cytoplasm. PKCbI is highly related to PKCbII. Unlike the mature PKCb II mRNA and protein, which rapidly increase following acute insulin treatment, the PKCbI mRNA and protein levels remain unchanged. Stable overexpression of PKCbII, but not PKCbI, leads to insulin-stimulated glucose uptake into cells. PKCb can mediate B-cell receptor signalling, and apoptosis induced by oxidative stress. PKCb can regulate oxidative stress inducing apoptosis, signalosome, B-cell activation, transcription regulation dependent on androgen receptor signalling, endothelial cell proliferation, and insulin secretion. PKCb can phosphorylate CARD11/CARMA1 at S559, S644, and S652. Upon stimulation of B cells and mast cells, Syk regulates Btk, and Btk selectively regulates enzymatic activity of PKCbI. Specific regulation of PKCbI by Btk is consistent with the selective association of Btk with PKCbI. PKCb has been linked with the development of diabetic nephropathy, colorectal adenocarcinomas and glioblastoma multiforme. PKCb may be an oncoprotein (OP), although it may also be a tumour suppressor protein, since it is actually down-regulated by phorbol esters and other tumour promoters. A higher Gleason Score significantly associated with increased PKCb expression in prostate cancer.

Figure 1. Dot blotting PKCb antibody with recombinant purified proteins.