Product Name: Akt2 (PKB beta)
Product Number: AB-NK130-3
Size: 25 µl      Price:89.00
      $US
Target Full Name: RAC-beta serine/threonine-protein kinase

Target Alias: AKT2; PKB; PKB beta; PRKBB; protein kinase Akt2; RAC BETA

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK130-3

Antibody Target Type: Pan-specific

Protein UniProt: P31751

Protein SigNET: P31751

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: A five residue synthetic peptide based on the human Akt2, coupled to KLH

Production Method: Affinity purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.25 mg/ml

Storage Buffer: TBS, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting, ELISA

Antibody Dilution Recommended: 1:3000 dilution was sufficient for detection of PKBβ in 20μg of HeLa cell lysate by ECL immunoblot analysis.

Antibody Potency: Very high potency. Detects a 65 kDa protein, corresponding to the molecular mass of Akt2 PKBβ on SDS-PAGE immunoblots.
Antibody Species Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.

Antibody Positive Control: A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Antibody Specificity: Seems very high

Antibody Cross Reactivity: Does not react with PKBα (Akt1) or PKBγ (Akt3).

Related Product 1: Akt2 pan-specific antibody (Cat. No.: AB-NK130-9P)

Related Product 2: Akt2-1 pan-specific antibody (Cat. No.: AB-NK130-4)

Related Product 3: Akt2-2 pan-specific antibody (Cat. No.: AB-NK130-8)

Related Product 4: Akt2-3 pan-specific antibody (Cat. No.: AB-NK130-9)

Related Product 5: PKB2-PCT (Akt2-4) pan-specific antibody (Cat. No.: AB-NK130-5)

Related Product 6: AktSubtide - Akt1 (PKBa) protein kinase substrate peptide

Related Product 7: GSK3b (3-12) KinSub - Glycogen synthase kinase 3-beta (GSK3b) N-terminus (G3-E12, human) peptide; Akt protein kinase substrate peptide

Scientific Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
Figure 1. Immunoblotting of various cell lines with AB-NK130-3 antibody at a 1 µg/mL final concentration. The target protein Akt2 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 57231.


Figure 2. Immunoblotting of various tissue lines with AB-NK130-3 antibody at a 1 µg/mL final concentration. The target protein Akt2 is indicated. Each lane was loaded with 15 µg of tissue lysate protein. The max signal count was 60367.


References

[1] Downward J (1998). Curr Opin Cell Biol. 10(2): 262-7.

[2] Brazil DP, and Hemmings BA (2001). Biochem Sci. 26(11):657-64.

[3] Nicholson KM, Anderson NG (2002). Cell Signal. 14(5): 381-95.

[4] Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.