Product Name: PAK1-NT (PAK1-1)
Product Number: AB-NK122-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: p21-activated kinase 1 alpha; Protein-serine/threonine kinase PAK 1

Target Alias: ADRB2; Alpha-PAK; CDC42,RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-A; Kinase PAK1; MUK2; P21 protein (Cdc42/Rac)-activated kinase 1; P65-PAK; STE20, yeast; P68-PAK; PAK 1; PAK-1; PAKA; Protein kinase MUK2; PAKalpha; MGC130000; MGC130001; CCDS8250.1; Q13153; ENSG00000149269

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK122-2

Antibody Target Type: Pan-specific

Protein UniProt: Q13153

Protein SigNET: Q13153

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PAK1 (PAKa) sequence peptide

Antibody Immunogen Sequence: MSNNGLDVQDKPC

Antibody Immunogen Description: Corresponds to amino acid residues M1 to P12; N-terminus

Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.25 mg/ml

Storage Buffer: 100 mM Tris-glycine, pH 7.0

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human; Mouse; Rat

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-67 kDa.

Antibody Cross Reactivity: Cross-reactivity with 60 kDa and 90 kDa proteins in various rat tissues.
Related Product 1: PAK1-pS144 phosphosite-specific antibody (Cat. No.: AB-PK748)

Related Product 2: PAK1-pT423 phosphosite-specific antibody (Cat. No.: AB-PK749)

Related Product 3: PAktide KinSub - PAK peptide substrate

Related Product 4: PAK1Subtide - PAK1 protein kinase substrate peptide

Scientific Background: PAK1 is a protein-serine/threonine kinase that is a member of the STE group of protein kinases in the STE20 family, and PAKA subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. PAK1 has been implicated in the regulation of cytoskeletal organization, and cell proliferation and survival, morphology, motility and transformation. Binding of GTP-bound Cdc42 or Rac1 to the autoregulatory region at the N-terminus of PAK1 releases monomers from the autoinhibited dimer, enables phosphorylation at T423 and activation of its phosphotransferase activity. It is also activated by binding to GTP-bound Cdc42, independent of the phosphorylation state of T423. Phosphorylation of S144, S149, and T423 increases PAK1's phosphotransferase activity, whereas phosphorylation of T84 (by OXSR1) and Y212 inhibits its phosphotransferase activity. Phosphorylation of S21 inhibits binding of Nck and PIX. PAK1 may be a therapeutic target in B-Raf wild-type melanoma. Knockdown of PAK1 has been shown to inhibit the proliferation of mutant KRAS colon cancer cells.