Product Name: Mos (Mos-1)
Product Number: AB-NK112
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Moloney sarcoma oncogene-encoded protein-serine kinase
Target Alias: ADRB2; C-mos; MGC119963; MSV; MGC119962; CCDS6164.1; ENSG00000172680
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK112
Antibody Target Type: Pan-specific
Protein UniProt: P00540
Protein SigNET: P00540
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Mos sequence peptide Cat. No.:
Antibody Immunogen Sequence: ASQRSFWAELNIARLRHDNIVRVVAASTRC
Target Alias: ADRB2; C-mos; MGC119963; MSV; MGC119962; CCDS6164.1; ENSG00000172680
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK112
Antibody Target Type: Pan-specific
Protein UniProt: P00540
Protein SigNET: P00540
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Mos sequence peptide Cat. No.:
Antibody Immunogen Sequence: ASQRSFWAELNIARLRHDNIVRVVAASTRC
Antibody Immunogen Description: Corresponds to amino acid residues A148 to R177; Kinase Catalytic Subdomain III
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity on Western blots with recombinant Xenopus laevis Mos expressed as a glutathione S-transferase fusion protein.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity on Western blots with recombinant Xenopus laevis Mos expressed as a glutathione S-transferase fusion protein.
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Cross Reactivity: Cross-reactivity with an unidentified rat protein of 60 kDa that is found in heart, liver and muscle.
Related Product 1: Mos-pY263 phosphosite-specific antibody (Cat. No.: AB-PK722)
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Cross Reactivity: Cross-reactivity with an unidentified rat protein of 60 kDa that is found in heart, liver and muscle.
Related Product 1: Mos-pY263 phosphosite-specific antibody (Cat. No.: AB-PK722)
Scientific Background: Mos is a protein-serine/threonine kinase of the Other group and Mos family. During oocyte maturation, it acts as a kinase that directly phosphorylates and activates MEK1 and MEK2 in the MAP kinase signalling module. It is also known as "cytostatic factor" in unfertilized oocytes. It is activated by phosphorylation at S3, and inhibited by phosphorylation at S102. Mos was originally identified as an oncoprotein from avial retroviral studies. While it phosphorylates and activates MEK1 and MEK2, there is little evidence that it functions as an oncoprotein in human cancers.
© Kinexus Bioinformatics Corporation 2017