Product Name: MEKK2-2
Product Number: AB-NK108-4
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: MAPK/ERK kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2
Target Alias: Kinase MEKK2; M3K2; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2B; ENSG00000169967
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK108-4
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y2U5
Protein SigNET: Q9Y2U5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEKK2 (MAP3K2) sequence peptide Cat. No.: PE-01AXU90
Target Alias: Kinase MEKK2; M3K2; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2B; ENSG00000169967
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK108-4
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y2U5
Protein SigNET: Q9Y2U5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEKK2 (MAP3K2) sequence peptide Cat. No.: PE-01AXU90
Antibody Immunogen Sequence: CLVINGSTQATNLEP
Antibody Immunogen Description: Corresponds to amino acid residues L119 to P132
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues L119 to P132
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 66-74 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEKK6 and YSK4.
Related Product 1: MEKK2-2 blocking peptide
Related Product 2: MEKK2-1 pan-specific antibody (Cat. No.: AB-NK108-3)
Related Product 3: MEKK2-3 pan-specific antibody (Cat. No.: AB-NK108-5)
Related Product 4: MEKK2-pS239 phosphosite-specific antibody (Cat. No.: AB-PK700)
Related Product 5: MEKK6-2 pan-specific antibody (Cat. No.: AB-NK225-2)
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEKK6 and YSK4.
Related Product 1: MEKK2-2 blocking peptide
Related Product 2: MEKK2-1 pan-specific antibody (Cat. No.: AB-NK108-3)
Related Product 3: MEKK2-3 pan-specific antibody (Cat. No.: AB-NK108-5)
Related Product 4: MEKK2-pS239 phosphosite-specific antibody (Cat. No.: AB-PK700)
Related Product 5: MEKK6-2 pan-specific antibody (Cat. No.: AB-NK225-2)
Scientific Background: MEKK2 (MAP3K2) is a protein-serine/threonine kinase of the STE group and STE11 family. It is required for cytokine production in cultured mast cells. The protein is one of the kinases that regulate different steps of the caveolar cycle. It activated by phosphorylation on T524. Methylation of the protein increases MAP kinase signalling and promotes Ras-driven carcinomas.
Figure 1. Dot blotting MEKK2-2 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017