Product Name: MEK5-1
Product Number: AB-NK104-3
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: MAPK/ERK protein-serine kinase 5 (MKK5); Dual specificity mitogen-activated protein kinase kinase 5
Target Alias: CXA1; HsT17454; MAP kinase kinase 5; MAP2K5; MAPK,ERK kinase 5; MAPK/ERK kinase 5; MAPKK5; MKK5; PRKMK5; HsT17454; ENSG00000137764
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK104-3
Antibody Target Type: Pan-specific
Protein UniProt: Q13163
Protein SigNET: Q13163
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK5 (MAP2K5, MKK5) sequence peptide Cat. No.: PE-01AVY90
Target Alias: CXA1; HsT17454; MAP kinase kinase 5; MAP2K5; MAPK,ERK kinase 5; MAPK/ERK kinase 5; MAPKK5; MKK5; PRKMK5; HsT17454; ENSG00000137764
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK104-3
Antibody Target Type: Pan-specific
Protein UniProt: Q13163
Protein SigNET: Q13163
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK5 (MAP2K5, MKK5) sequence peptide Cat. No.: PE-01AVY90
Antibody Immunogen Sequence: CAELKKILANGQMNEQ
Antibody Immunogen Description: Corresponds to amino acid residues A150 to Q164
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues A150 to Q164
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-52 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEK1, MKK4 and MKK6.
Related Product 1: MEK5-1 blocking peptide
Related Product 2: MEK5-2 pan-specific antibody (Cat. No.: AB-NK104-4)
Related Product 3: MEK5-3 pan-specific antibody (Cat. No.: AB-NK104-5)
Related Product 4: MEK5-PNT (MEK5-4) pan-specific antibody (Cat. No.: AB-NK104-2)
Related Product 5: MEK5-pS311 phosphosite-specific antibody (Cat. No.: AB-PK699)
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEK1, MKK4 and MKK6.
Related Product 1: MEK5-1 blocking peptide
Related Product 2: MEK5-2 pan-specific antibody (Cat. No.: AB-NK104-4)
Related Product 3: MEK5-3 pan-specific antibody (Cat. No.: AB-NK104-5)
Related Product 4: MEK5-PNT (MEK5-4) pan-specific antibody (Cat. No.: AB-NK104-2)
Related Product 5: MEK5-pS311 phosphosite-specific antibody (Cat. No.: AB-PK699)
Scientific Background: MEK5 (MAP2K5) is a Dual specificity protein kinase of the STE group and STE7 family. It is activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis by acting as a scaffold protein for the construction of a complex consisting of MAP3K2 or MAP3K3 and MAP3K5 and MAPK7.
Figure 1. Dot blotting MEK5-1 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017