Product Name: MKK3-1
Product Number: AB-NK101-4
Size: 25 µg      Price:89.00
      $US
Target Full Name: MAPK/ERK protein-serine kinase 3 beta isoform (MKK3 beta); Dual specificity mitogen-activated protein kinase kinase 3

Target Alias: MAP kinase kinase 3; MAPK,ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAP2K3; MP2K3; PRKMK3; P46734; ENSG00000034152

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK101-4

Antibody Target Type: Pan-specific

Protein UniProt: P46734

Protein SigNET: P46734

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MKK3 (MAP2K3, MEK3) sequence peptide Cat. No.: PE-01AWB99

Antibody Immunogen Sequence: SRTFITIGDRNFEVEA

Antibody Immunogen Description: Corresponds to amino acid residues S46 to A61

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human; Mouse

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-40 kDa.

Antibody Specificity: Medium

Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEK1, MKK4 and MKK6.

Related Product 1: MKK3-1 blocking peptide

Related Product 2: MKK3-2 pan-specific antibody (Cat. No.: AB-NK101-5)

Related Product 3: MKK3-3 pan-specific antibody (Cat. No.: AB-NK101-6)

Related Product 4: MKK3-pS218 phosphosite-specific antibody (Cat. No.: AB-PK713)

Related Product 5: MKK3-pY230 phosphosite-specific antibody (Cat. No.: AB-PK714)

Scientific Background: MKK3 (MAP2K3, MEK3) is a Dual specificity protein kinase of the STE group and STE7 family. MKK3 is activated by cytokines and environmental stress in vivo. Phosphorylation of S218 and T222 increases its phosphotransferase activity. S218A and T222A substitutions are involved in inactivation. S218E and T222E mutations are associated in constitutive activation. It catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the activation T loops of the p38 MAP kinase isoforms. Defects in the MKK3 gene may be involved colon cancer.
Figure 1. Dot blotting MKK3-1 antibody with recombinant purified proteins.