Product Name: GSK-3Beta
Product Number: AB-NK070-4
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Glycogen synthase kinase-3 beta
Target Alias: GSK 3 beta; GSK 3B; GSK-3 beta; GSK3B; GSK3B_Human; GSK3beta isoform
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK070-4
Antibody Target Type: Pan-specific
Protein UniProt: P49841
Protein SigNET: P49841
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
Target Alias: GSK 3 beta; GSK 3B; GSK-3 beta; GSK3B; GSK3B_Human; GSK3beta isoform
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK070-4
Antibody Target Type: Pan-specific
Protein UniProt: P49841
Protein SigNET: P49841
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.3 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.3 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~47 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine
Antibody Positive Control: 1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Related Product 1: GSK3b-ANT pan-specific antibody (Cat. No.: AB-NK270-3)
Related Product 2: GSK3b-XI pan-specific antibody (Cat. No.: AB-NK194-NK195-3)
Related Product 3: GSK3b-XI (GSK3b-1) pan-specific antibody (Cat. No.: AB-NK070)
Antibody Species Reactivity: Human | Mouse | Rat | Bovine
Antibody Positive Control: 1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Medium
Related Product 1: GSK3b-ANT pan-specific antibody (Cat. No.: AB-NK270-3)
Related Product 2: GSK3b-XI pan-specific antibody (Cat. No.: AB-NK194-NK195-3)
Related Product 3: GSK3b-XI (GSK3b-1) pan-specific antibody (Cat. No.: AB-NK070)
Related Product 4: GSK3a-pS278+pY279 phosphosite-specific antibody (Cat. No.: AB-PK647)
Related Product 5: GSK3a-pT19+pS21 phosphosite-specific antibody (Cat. No.: AB-PK648)
Related Product 6: GSK3a-pY279 phosphosite-specific antibody (Cat. No.: AB-PK649)
Related Product 7: GSK3a-pY284+Y285 phosphosite-specific antibody (Cat. No.: AB-PK650)
Related Product 8: GSK3Selectide - GSK3 protein kinase substrate peptide
Related Product 9: GSK3Selectide - GSK3 protein kinase substrate peptide
Related Product 5: GSK3a-pT19+pS21 phosphosite-specific antibody (Cat. No.: AB-PK648)
Related Product 6: GSK3a-pY279 phosphosite-specific antibody (Cat. No.: AB-PK649)
Related Product 7: GSK3a-pY284+Y285 phosphosite-specific antibody (Cat. No.: AB-PK650)
Related Product 8: GSK3Selectide - GSK3 protein kinase substrate peptide
Related Product 9: GSK3Selectide - GSK3 protein kinase substrate peptide
Scientific Background: Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues. It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3). In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta. They are structurally similar, but functionally non-identical. GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4). GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer’s, cancer and bipolar (4, 5).
References
[1] Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595.
[2] Wang H. (2011) Cytokind. 53(2): 130-140.
[3] Mills C.N. (2011) Front Mol Neurosci. 47(4): 47.
[4] Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063.
[5] Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.
[1] Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595.
[2] Wang H. (2011) Cytokind. 53(2): 130-140.
[3] Mills C.N. (2011) Front Mol Neurosci. 47(4): 47.
[4] Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063.
[5] Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.
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