Product Name: CaMKK1 (CaMKK1-1)
Product Number: AB-NK022
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase kinase
Target Alias: Calcium/calmodulin-dependent protein kinase 1 alpha, isoform a; Calcium/calmodulin-dependent protein kinase kinase 1, alpha; CaMKK alpha protein; CaMKK1.; CaMKKA; DKFZP761M0423; KKCC1; MGC34095
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK022
Antibody Target Type: Pan-specific
Protein UniProt: Q8N5S9
Protein SigNET: Q8N5S9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMKK1 (CaMKK) sequence peptide
Target Alias: Calcium/calmodulin-dependent protein kinase 1 alpha, isoform a; Calcium/calmodulin-dependent protein kinase kinase 1, alpha; CaMKK alpha protein; CaMKK1.; CaMKKA; DKFZP761M0423; KKCC1; MGC34095
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK022
Antibody Target Type: Pan-specific
Protein UniProt: Q8N5S9
Protein SigNET: Q8N5S9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMKK1 (CaMKK) sequence peptide
Antibody Immunogen Sequence: CGKSPELPGVQEDEA
Antibody Immunogen Description: Corresponds to amino acid residues G490 to A503; C-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues G490 to A503; C-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Rat
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Cross Reactivity: Cross-reacts with additional cross-reactive proteins in various rat tissues.
Related Product 1: CaMKK1-pS74 phosphosite-specific antibody (Cat. No.: AB-PK557)
Related Product 2: CaMKK1-pS74 phosphosite-specific antibody (Cat. No.: AB-PK557)
Antibody Cross Reactivity: Cross-reacts with additional cross-reactive proteins in various rat tissues.
Related Product 1: CaMKK1-pS74 phosphosite-specific antibody (Cat. No.: AB-PK557)
Related Product 2: CaMKK1-pS74 phosphosite-specific antibody (Cat. No.: AB-PK557)
Scientific Background: CaMKK1 is a protein-serine/threonine kinase of the Other group and CaMKK family. It is a calcium/calmodulin-activated protein kinase that phosphorylates and activates CaMK1, CaMK1d, CaMK1g, CaMK4 and Akt1. Binding of calmodulin may releave intrasteric autoinhibition. It is partially inhibited upon phosphorylation by PRCAKA/PKA
Figure 1. Dot blotting CaMKK1-1 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017