Product Name: CaMPK4-NT (CaMK4-2 ?)

Product Number: AB-NK021-1

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 4

Target Alias: Brain Ca++-calmodulin-dependent protein kinase type IV; Calcium/calmodulin-dependent protein kinase IV; Calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calspermin; CAM kinase- GR; CAM kinase IV; CAM kinase-GR; CaMK IV; CaMK4; Kinase CaMK4; CaMK-GR; CaMKIV; KCC4 CaMK IV; MGC36771; CCDS4103.1; ENSG00000152495

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK021-1

Antibody Target Type: Pan-specific

Protein UniProt: Q16566

Protein SigNET: Q16566

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CaMK4 (CaMPK4) sequence peptide

Antibody Immunogen Sequence: CSSVTSSTENLVPDYWID

Antibody Immunogen Description: Corresponds to amino acid residues C14 to D31; N-terminus

Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: 100 mM Tris-glycine, pH 7.0

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity on Western blots with recombinant mouse calmodulin-dependent protein kinase 4 expressed in sf9 insect cells.

Antibody Species Reactivity: Mouse; Rat

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.

Scientific Background: CaMK4 is a protein-serine/threonine kinase of the CaMK group and CaMK1 family. It is a calcium/calmodulin-dependent protein kinase. This kinase is monomeric in structure and occurs in two isoforms generated by alternative splcing from the same gene. It is implicated in immune response, inflammation, memory consolidation, positive selection of T lymphocytes, cytokine production, regulation in neurons and male germ cells, survival and differentiation of osteoblasts and dendritic cells. It is highly expressed in rat tissues such as the forebrain, lymphocytes, spleen, testis, and thymus. Immunohistochemical studies have demonstrated a nuclear localization of the transiently expressed CaMK4. The cytoskeletal and perinuclear localization of inducible CaMK4 implies that the kinase may be a modulator of cell shape and/or motility and/or activity of a cytoskeleton-associated nuclear factor. It regulates many transcription activators such as CREB1, and JUN. It can also activate MAPK pathway through phosphorylation of ELK1 and ATF2. Mutations (S12A, S13A, T57A+K58E, K75E, T200A) are mostly result in loss of phosphotransferase activity or loss of activation by the upstream kinases CaMKK1 or CaMKK2. Some mutations (S189A, H309D+M310E+T312D+F320D+N321D+) lead to a constitutively active form.

Figure 1. Dot blotting CaMK4-2 antibody with recombinant purified proteins.