Product Name: CaMK2d-NT
Product Number: AB-NK019-3
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 delta
Target Alias: Calcium/calmodulin-dependent protein kinase II delta; Calcium/calmodulin-dependent protein kinase type II delta chain; CaM kinase II delta; CaMK2-delta; CaMKD; CaMK-II delta; CaM-kinase II delta; KCC2D; KCCD; MGC44911; CCDS3703.1; ENSG00000145349
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK019-3
Antibody Target Type: Pan-specific
Protein UniProt: Q13557 Protein SigNET: Q13557 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK2d sequence peptide Cat. No.: PE-01BCI85
Antibody Immunogen Sequence: CTRFTDEYQLFEELGKGAFS
Antibody Immunogen Description: Corresponds to amino acid residues C7 to S26; N-terminus and kinase domain I, highly conserved in all CaMK2 isoforms
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Related Product 1: CaMK2d-NT blocking peptide Scientific Background: CaMK2d is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-activated protein kinase. It is activated in response to a rise in intracellular Ca(2+) concentration. Autophosphorylation at Thr-287 probably increases phosphotransferase activity in a Ca(2+)-independent manner.
Figure 1. Dot blotting CaMK2d-NT antibody with recombinant purified proteins.