Product Name: CaMK2b-PAD

Product Number: AB-NK018-3

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 beta

Target Alias: Calcium/calmodulin-dependent protein kinase II beta; Calcium/calmodulin-dependent protein kinase type II beta chain; CaM kinase II beta subunit; CAM2; CaMK2; CaMK2-beta; CaMKB; CaMK-II beta; CaMK-II beta subunit; Proline rich calmodulin-dependent protein kinase; CaM-kinase II beta chain; KCC2B; Kinase CaMK2-beta; MGC29528; CCDS5483.1; ENSG00000058404

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK018-3

Antibody Target Type: Pan-specific

Protein UniProt: Q13554

Protein SigNET: Q13554

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CaMK2b sequence peptide Cat. No.: PE-01BCH85

Antibody Immunogen Sequence: RISDILNSVRRGSGTPEAEC

Antibody Immunogen Description: Corresponds to amino acid residues R449 to E467; Before CaMAD, repeated 3X

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.

Related Product 1: CaMK2b-PAD blocking peptide

Scientific Background: CaMK2b is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-dependent protein kinase. Autophosphorylation at T287 increases phosphotransferase activity in a Ca(2+)-independent manner. It is an important kinase in the central nervous system and functions as components in long-term potentiation and neurotransmitter release. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplamic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2 isoforms, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. CaMK2b participates in the regulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates to the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

Figure 1. Dot blotting CaMK2b-PAD antibody with recombinant purified proteins.